| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.200976 |
| Chromosome: | chromosome_6 |
| Location: | 3932691 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278186 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAATGCGTACAATATGCAATGGCTTCTCA |
| Internal bar code: | CGAATGCGTTCGTATTCACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 99.5851 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGGCTGAAAGATGGTC |
| Suggested primer 2: | GCATGACAGCAAGTGCCTTA |