Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.201017 |
Chromosome: | chromosome_2 |
Location: | 3705770 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre02.g095150 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAAGAGCTTGTGCACAGGGAGAAGGGGGG |
Internal bar code: | CTGTAGTCACCGGAACTGACGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 645 |
LEAP-Seq percent confirming: | 99.7001 |
LEAP-Seq n confirming: | 1330 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GATCCGACGTTAGCTTCGAC |
Suggested primer 2: | ATACGCCAGTAAGCAAGCGT |