| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201036 |
| Chromosome: | chromosome 5 |
| Location: | 413983 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232800 | SRX1 | Sulfiredoxin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGCGGGCGACACGCGAATGAGCTTG |
| Internal bar code: | TCCAGTGAAAACACTTAGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 99.6197 |
| LEAP-Seq n confirming: | 3143 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTCATGCCAACATCCA |
| Suggested primer 2: | TGCATCAGTGCGTGTGTAAA |