| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201087 |
| Chromosome: | chromosome_2 |
| Location: | 2871603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g094801 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCACGGGTGCTCGCGTGGCCCTGCCTGC |
| Internal bar code: | GCCGGCCCACGCTGCAGGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 994 |
| LEAP-Seq percent confirming: | 99.166 |
| LEAP-Seq n confirming: | 4756 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACGGCGATTTCATCAAT |
| Suggested primer 2: | CGACGTTGAGGTAGGACCAT |