| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201130 |
| Chromosome: | chromosome 16 |
| Location: | 3609125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683793 | (1 of 1) K14843 - pescadillo (PES1, NOP7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGTCCCAAATCCTTACTCACACATTTT |
| Internal bar code: | TGGATGATTTTCAAGGCGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCAGAGTCCAGCCGTC |
| Suggested primer 2: | TGGCAGATAAAACTACCGCC |