| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201148 |
| Chromosome: | chromosome 16 |
| Location: | 2124753 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657950 | SSA5 | cilia-sensing, structure and/or assembly; (1 of 1) K06259 - CD36 antigen (CD36) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCAATTAAGGAAGTGAATGCGTAGCC |
| Internal bar code: | GGGGGACGAGCCGGGATGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 97.9107 |
| LEAP-Seq n confirming: | 3421 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTCTGAAACATTCGCTT |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |