| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201153 |
| Chromosome: | chromosome 11 |
| Location: | 1309270 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467711 | (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTAGGCCCTGTGACGTCCCTGTCCCTA |
| Internal bar code: | GGCCGATGCTTTAGGTGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 26 |
| LEAP-Seq percent confirming: | 57.5 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAAGCTCCAAGAGATGC |
| Suggested primer 2: | GCTCGTAGGCCTGAGATTTG |