| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201188 |
| Chromosome: | chromosome 14 |
| Location: | 881672 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613501 | ZXE5 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED; Putative zeaxanthin epoxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGAGGCAGGGCAGGAGGTGGCGGAGCG |
| Internal bar code: | GAGGACTACTTGCGCCAAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 99.0924 |
| LEAP-Seq n confirming: | 1201 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCAGACACTTCCTGTGA |
| Suggested primer 2: | CGGCGGTTAGCTAGTAGCAC |