| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201207 |
| Chromosome: | chromosome 10 |
| Location: | 2798188 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439100 | CCT1 | T-complex protein 1, alpha subunit; (1 of 1) K09493 - T-complex protein 1 subunit alpha (CCT1, TCP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCAGGTCGTCGATGCGCAGGATGGTGA |
| Internal bar code: | TCTGCTTAAGGTTAACTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 692 |
| LEAP-Seq percent confirming: | 99.7722 |
| LEAP-Seq n confirming: | 2628 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGGAGGAGATGGACTGA |
| Suggested primer 2: | CTTGAGATAGCATGCCGTGA |