Insertion junction: LMJ.RY0402.201210_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201210
Chromosome:chromosome 17
Location:7043364
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g746747 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCCGCACTCGATATGCAGTAATGAACGC
Internal bar code:GGGAGTCCGACCGACAGCTGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:99.64
LEAP-Seq n confirming:1107
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTACAAGAGGGACCTGC
Suggested primer 2:TGGCACTTTGTTTCCTACCC