| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201218 |
| Chromosome: | chromosome 9 |
| Location: | 6273864 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406432 | 5'UTR|outside_mRNA | ||
| Cre09.g406448 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCGGCCAGCCAGCAGCAGTAATAACCG |
| Internal bar code: | GAGCGCATCTGCCAAGGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 58.5366 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCACGGTCATGTTTAGA |
| Suggested primer 2: | AGGTGTACCGGATGAGAACG |