| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201219 |
| Chromosome: | chromosome 1 |
| Location: | 3319302 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020918 | PREP1 | (1 of 1) PTHR11851:SF68 - PRESEQUENCE PROTEASE 1, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Presequence protease 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTGCAACTGCACCTGCCTACGACTTCA |
| Internal bar code: | GGCCCTTAATTTGCGACGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 99.12 |
| LEAP-Seq n confirming: | 2140 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCTCCACTTCCTCCACAC |
| Suggested primer 2: | GGAGCTCTACTCATGCACCC |