| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201228 |
| Chromosome: | chromosome 6 |
| Location: | 7990591 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303900 | (1 of 1) PTHR20855:SF52 - ADIPONECTIN RECEPTOR PROTEIN | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGATGGCAGGTCCATGGGATGGAGGA |
| Internal bar code: | GTCCAGCCGACGGCATACGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 99.8056 |
| LEAP-Seq n confirming: | 2054 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTTTTTGATGGCTTGTTT |
| Suggested primer 2: | CATTACGGTAGTGGCGAGGT |