Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.201236 |
Chromosome: | chromosome_13 |
Location: | 355473 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g564400 | FAP161 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGCGTGTACACTGGCAGGGCAGCTAGGAGG |
Internal bar code: | CGGGTTACAGATGGCCGCGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 343 |
LEAP-Seq percent confirming: | 95.7895 |
LEAP-Seq n confirming: | 91 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGAGACACCCATTCCACCT |
Suggested primer 2: | GTGCCTCTCATCGAAAAAGC |