| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201262 |
| Chromosome: | chromosome 10 |
| Location: | 434417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420700 | ATP15 | Mitochondrial F1F0 ATP synthase, epsilon subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCTATCGACCGCCCGCACGAACCCACC |
| Internal bar code: | TCCGGCACCCTTCGCGGTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 829 |
| LEAP-Seq percent confirming: | 99.1083 |
| LEAP-Seq n confirming: | 1556 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACGTTGGTCATGGACT |
| Suggested primer 2: | CAGACCGTCTACTTCCGCTC |