| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201275 |
| Chromosome: | chromosome_10 |
| Location: | 1655252 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g429750 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTATGGCACTTGATTGGTTTTACGTTCCT |
| Internal bar code: | CAACTCCGCCGGAACAAAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 99.8646 |
| LEAP-Seq n confirming: | 1475 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGGTGTGTCGAGTACGG |
| Suggested primer 2: | GCAACTGACAGTACAGCCCA |