| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201280 |
| Chromosome: | chromosome 6 |
| Location: | 8828534 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g310450 | (1 of 1) K03124 - transcription initiation factor TFIIB (TFIIB, GTF2B, SUA7, tfb) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGGCTTAGGCAGGAGGCAGCAAGTCAC |
| Internal bar code: | TCGTGTCACTGGTTCGGCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.602 |
| LEAP-Seq n confirming: | 1001 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTAAGACAGCTGGCACG |
| Suggested primer 2: | AACCCGGTCAACAAGACAAG |