| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201280 |
| Chromosome: | chromosome 12 |
| Location: | 3715682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514700 | PPHC1,PPH | Pheophytinase 1, chloroplastic; (1 of 1) PTHR10992:SF879 - PHEOPHYTINASE, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTGAATGATGTGGGGACGTTCTGCCA |
| Internal bar code: | CGGCCGCCCCGGGCGGGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.8369 |
| LEAP-Seq n confirming: | 1224 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTGTGGTGTGGAGTGAC |
| Suggested primer 2: | CCCTCTATGTGGCTGATGGT |