| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201287 |
| Chromosome: | chromosome 3 |
| Location: | 6157638 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192050 | FTR1 | (1 of 1) K07243 - high-affinity iron transporter (FTR, efeU); Iron permease 1, membrane protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAAAAAGACGATGTCGCTAGTTCGAG |
| Internal bar code: | TAGCGAAGCACACGGGGATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 876 |
| LEAP-Seq percent confirming: | 99.7653 |
| LEAP-Seq n confirming: | 3826 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCCGACTACAAGCACAA |
| Suggested primer 2: | GATTCTGACCGACCTTTCCA |