| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201309 |
| Chromosome: | chromosome 3 |
| Location: | 3276647 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165900 | PGM17 | (1 of 3) 3.1.3.46 - Fructose-2,6-bisphosphate 2-phosphatase / Fructose-2,6-bisphosphatase; Putative phosphoglycerate mutase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGAACCTCCACCCTCCCCTCCCCCAGCC |
| Internal bar code: | AGTGTCCAAGTGTTTCCGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 21.7054 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 202 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAGGGACTACCAAG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |