| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201310 |
| Chromosome: | chromosome 1 |
| Location: | 5298909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037050 | (1 of 3) 2.4.2.30 - NAD(+) ADP-ribosyltransferase / Poly(ADP-ribose)polymerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACTGGATTAGTGTTGACACTGGCCATCT |
| Internal bar code: | AACAGGAGTTCTATGGTGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 526 |
| LEAP-Seq percent confirming: | 99.6454 |
| LEAP-Seq n confirming: | 562 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATCGAGAAGGTGATGGC |
| Suggested primer 2: | CTGAGCTCACTTCTTTGGGG |