| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201319 |
| Chromosome: | chromosome 3 |
| Location: | 1934662 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155400 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAGACGCCGTTCGTGACTTCCCCAGC |
| Internal bar code: | CCTTGTGGGATACTCTCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 99.5015 |
| LEAP-Seq n confirming: | 998 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGGCCTTGCCTCTCTTA |
| Suggested primer 2: | GCTAGACAACTCCGACTCCG |