| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201330 |
| Chromosome: | chromosome 3 |
| Location: | 3124409 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164950 | SRS3 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 5) IPR000504//IPR012677 - RNA recognition motif domain // Nucleotide-binding alpha-beta plait domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCGTGCGGCTCCCAGTTGCGCTGCAAAT |
| Internal bar code: | GGTTAGGCTGCTGACCGTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 361 |
| LEAP-Seq percent confirming: | 98.8764 |
| LEAP-Seq n confirming: | 176 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTTGAGCATCCACTTC |
| Suggested primer 2: | TCGCTGCACCTTCCTAACTT |