Insertion junction: LMJ.RY0402.201343_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.201343
Chromosome:chromosome 8
Location:3328157
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g373916 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGTTTGGGTCGAAGCGGCCGCTGGACAT
Internal bar code:ACAAGAAGAGCAACTAATTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:99.7159
LEAP-Seq n confirming:1404
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AAATCCCTCACAAGCACCAC
Suggested primer 2:ACACACACACACACACACGC