| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201360 |
| Chromosome: | chromosome 1 |
| Location: | 6926856 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049900 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCAGGAGTCTCCAATCCTGACTGCCA |
| Internal bar code: | GTGTGGAGTGGCGGATCAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 926 |
| LEAP-Seq percent confirming: | 85.2522 |
| LEAP-Seq n confirming: | 3769 |
| LEAP-Seq n nonconfirming: | 652 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAGGAGGCACCGCTAGAA |
| Suggested primer 2: | CTAGAGACCGGAGGATGCAG |