| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201453 |
| Chromosome: | chromosome 5 |
| Location: | 883364 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247550 | GST7 | (1 of 1) PTHR11260:SF220 - GST-CONTAINING FLYWCH ZINC-FINGER PROTEIN; Glutathione S-transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGCATAGACCGCTGTGTTCCGCTTCGG |
| Internal bar code: | GAGCGTGTCTCGGAGGCCGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1015 |
| LEAP-Seq percent confirming: | 99.673 |
| LEAP-Seq n confirming: | 1829 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGTGTATTGCGTGCGAG |
| Suggested primer 2: | GGAGGCAGAATCGTTCAGAG |