| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201460 |
| Chromosome: | chromosome 12 |
| Location: | 7873766 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552851 | OGT1 | (1 of 1) PTHR23083//PTHR23083:SF397 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED; O-linked N-acetylglucosamine transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGTTGGGGATTACGGATAACGAGTGTA |
| Internal bar code: | CATTGCTGGGCCTAACGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.7602 |
| LEAP-Seq n confirming: | 7903 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGAGTGAGTGGGTGGGT |
| Suggested primer 2: | AGAAGAAGGATGTGGATGCG |