| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201478 |
| Chromosome: | chromosome 13 |
| Location: | 3414279 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTCTGGTGCTCTAATGCATGCATGTCC |
| Internal bar code: | GGCATGCACAAGTCCGGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 939 |
| LEAP-Seq percent confirming: | 98.5141 |
| LEAP-Seq n confirming: | 663 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGAGCGGTTCTCGGTAAG |
| Suggested primer 2: | GTTGGCATACAGAGTCCCGT |