| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201550 |
| Chromosome: | chromosome 1 |
| Location: | 7506711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054000 | (1 of 3) PTHR23149:SF9 - PIN2/TERF1-INTERACTING TELOMERASE INHIBITOR 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGATGCTGTCCGGCGCTGCGCTGTG |
| Internal bar code: | TCTCCACTGTCTGGCGCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 94.9095 |
| LEAP-Seq n confirming: | 2778 |
| LEAP-Seq n nonconfirming: | 149 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTCAACCAGCCAGCTCT |
| Suggested primer 2: | TGACAGCCTCGTCTGTGAAC |