| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201552 |
| Chromosome: | chromosome 2 |
| Location: | 273629 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074950 | (1 of 5) PTHR10351//PTHR10351:SF42 - TRANSCRIPTION FACTOR BTF3 // LD40663P | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCGGCGTTACCAGGTGGCCTGTCAGCG |
| Internal bar code: | ATAGGCCTGGCTTATCCCACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 87.4688 |
| LEAP-Seq n confirming: | 1403 |
| LEAP-Seq n nonconfirming: | 201 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGACAGGAACAAGGTGGG |
| Suggested primer 2: | TTACTGCTCATCACGCCAAG |