| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201556 |
| Chromosome: | chromosome 15 |
| Location: | 1765612 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g643500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGCTTGTGCGATCGGTCCCTCCCACACG |
| Internal bar code: | TCGACGGCTGCAGCCCGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.8517 |
| LEAP-Seq n confirming: | 2694 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCATCCTTCTTAGGGC |
| Suggested primer 2: | ATAATGTGAGGTTGCGAGGG |