Insertion junction: LMJ.RY0402.201565_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201565
Chromosome:chromosome 9
Location:5011088
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g398067 FKB62,ROF1 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516:SF324 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP62 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATGAACACAGAAAGTTCGACCCATCGA
Internal bar code:GCAAGGATGAATGCGGACCGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:41.8647
LEAP-Seq n confirming:458
LEAP-Seq n nonconfirming:636
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGTGATCAACCCCCAAC
Suggested primer 2:CCACCGTGTTCTCATTGTTG