| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201586 |
| Chromosome: | chromosome 8 |
| Location: | 678454 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g360100 | DUR2 | Allophanate hydrolase; (1 of 1) PTHR11895//PTHR11895:SF24 - AMIDASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAACTACACGACCCGCCACCGCCGCCG |
| Internal bar code: | ATGCGGCTCAGTAATGACGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 260 |
| LEAP-Seq percent confirming: | 98.2353 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGTGGTTGTACACGTC |
| Suggested primer 2: | AAAGACACCAGAACCCCTCC |