| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201591 |
| Chromosome: | chromosome 11 |
| Location: | 3781032 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g483250 | PHC45 | (1 of 8) 3.1.1.13//3.1.1.3 - Sterol esterase / Triterpenol esterase // Triacylglycerol lipase / Triglyceride lipase; Putative pherophorin-chlamydomonas homolog | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCACCACCCGCACAATTGCCCAGCCCGC |
| Internal bar code: | GCCTGGCCTGGACGAGGGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 219 |
| LEAP-Seq percent confirming: | 92.2727 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCACCCACCTGTCTAC |
| Suggested primer 2: | GGGAGTAGGAGGCGAGGAC |