| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201602 |
| Chromosome: | chromosome 16 |
| Location: | 3531930 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678452 | (1 of 1) PF09753 - Membrane fusion protein Use1 (Use1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGGGCGAAAATGGGTGCTTCGTTACC |
| Internal bar code: | AAAGGCGCCTTGGCACACGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 95.0704 |
| LEAP-Seq n confirming: | 405 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTGGCTACCTGTTGTG |
| Suggested primer 2: | ACTCATTCTTGTTCCACGCC |