Insertion junction: LMJ.RY0402.201610_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201610
Chromosome:chromosome_9
Location:3671715
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g390615 FAP12 Triacylglycerol lipase and Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCCGCGCCGCACGCCGCACGTCGGCACGTG
Internal bar code:TTTGGAGTCTAGGTGGAGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:853
LEAP-Seq percent confirming:99.8261
LEAP-Seq n confirming:2871
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGCCCTCCATCCTGAGTT
Suggested primer 2:CAGGTTTGCTGGATTGGATT