| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201610 |
| Chromosome: | chromosome_10 |
| Location: | 81973 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g418150 | FAP178 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCACTAGGAAGCCGTTCGCCACGTCCCTG |
| Internal bar code: | ATCCGTGACCGGTCATTAGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 99.9104 |
| LEAP-Seq n confirming: | 2229 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCAGTCCGGCTGTGTT |
| Suggested primer 2: | CAAAGCTTCGCAATCACAAA |