| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201623 |
| Chromosome: | chromosome 16 |
| Location: | 4086635 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669650 | (1 of 2) 3.5.99.10 - 2-iminobutanoate/2-iminopropanoate deaminase / Enamine/imine deaminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCAAGGGCAGTGCTCGCATCTCTCGCC |
| Internal bar code: | GACGGTGCAGACGGCGCGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.8871 |
| LEAP-Seq n confirming: | 885 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCGATGGTTTTGATGTG |
| Suggested primer 2: | CATGTGGGGGTATTTGGAAG |