| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201634 |
| Chromosome: | chromosome 15 |
| Location: | 482585 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636650 | (1 of 1) PF12251 - snRNA-activating protein of 50kDa MW C terminal (zf-SNAP50_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCGGTCCGGGTGCGTGTGTGTGTGTG |
| Internal bar code: | CCGTGCGCCGTGCGGCCCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 117 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCCATGAACGGCTATAC |
| Suggested primer 2: | AAACGGAACCTTTGTCATGC |