| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201648 |
| Chromosome: | chromosome_16 |
| Location: | 1627319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTGGCCCGCAGCGTGCGACCATATTTAGT |
| Internal bar code: | GCTTTTCGCCTGCGGGCGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 99.8586 |
| LEAP-Seq n confirming: | 706 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCTAGCAGGTCCTCAG |
| Suggested primer 2: | TCTAGCAGCTTCCTACCGGA |