| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.201649 |
| Chromosome: | chromosome 16 |
| Location: | 1419515 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652300 | CMA2,TTL13 | Chlamydomonas-specific membrane protein family A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCTGTAACAAAACCAATGTACCGGTTT |
| Internal bar code: | GTGAGTCGTGCATGGACCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 |
| LEAP-Seq percent confirming: | 75.4601 |
| LEAP-Seq n confirming: | 369 |
| LEAP-Seq n nonconfirming: | 120 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTGTGACCTGCATTTG |
| Suggested primer 2: | ATCTATCCCATGGGGTCTCC |