Insertion junction: LMJ.RY0402.201653_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.201653
Chromosome:chromosome 17
Location:2701117
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATTAATGCTGCTCCTAGCAAAAACCGCGA
Internal bar code:CACTACAGCGATCGGGTATCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:452
LEAP-Seq percent confirming:99.7638
LEAP-Seq n confirming:3379
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTTCACGTTGAACTGAGG
Suggested primer 2:CGTTTCGTCTTGGTCCAAAT