| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.201675 |
| Chromosome: | chromosome 9 |
| Location: | 5478937 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400293 | (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGCACCAGAACCGGGGCTGTACCAGG |
| Internal bar code: | CGCTTTGTTACGTTTAATAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.1379 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCCAGGTTGATGTGCTC |
| Suggested primer 2: | CTTCTACAGCCCCACTGAGC |