| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201682 |
| Chromosome: | chromosome 14 |
| Location: | 1262782 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616550 | CYG50 | (1 of 1) IPR000104//IPR001054//IPR006189//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // CHASE domain // Nucleotide cyclase; Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGTACAGTCCATACTCAATTCTGCCA |
| Internal bar code: | GCAGTACAGGGCAGGTATCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 98.3757 |
| LEAP-Seq n confirming: | 1393 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTATGAGAACACGTCCCG |
| Suggested primer 2: | ATGTAGCCAGCACGACTGTG |