| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.201692 |
| Chromosome: | chromosome 10 |
| Location: | 3665264 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445850 | MAE14 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF); MATE efflux family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGTAACCAAGTTACATGCCCCCGGCG |
| Internal bar code: | CCTAAGTGGTGGCCGAGTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 99.1597 |
| LEAP-Seq n confirming: | 236 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCATGGCTTTGTACTCC |
| Suggested primer 2: | AGCTATTGCACGGTTATGGC |