Insertion junction: LMJ.RY0402.201696_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.201696
Chromosome:chromosome 3
Location:6743319
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g198000 PPP15 (1 of 2) PF00481//PF13672 - Protein phosphatase 2C (PP2C) // Protein phosphatase 2C (PP2C_2); Phosphoprotein phosphatase 2C-related 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAATCAGATCTGAGGGTGGCATAGCGCC
Internal bar code:ATTTCCCGCGTGCAATAAGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:584
LEAP-Seq percent confirming:99.3464
LEAP-Seq n confirming:152
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCTGGCAGTTATGGGTG
Suggested primer 2:CCACCCCCTGTTCAACTCTA