| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201722 |
| Chromosome: | chromosome 17 |
| Location: | 1308348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705778 | Chlamydomonas-specific family W protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGCATCACGCACGTACTTGGCACCCGC |
| Internal bar code: | ATTCGTGGTACCACCCGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2778 |
| LEAP-Seq percent confirming: | 73.6384 |
| LEAP-Seq n confirming: | 2623 |
| LEAP-Seq n nonconfirming: | 939 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCACAGCACAGCATAGA |
| Suggested primer 2: | GTCCACATGCTCTCAAGGGT |