Insertion junction: LMJ.RY0402.201747_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201747
Chromosome:chromosome 6
Location:8222573
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g305650 PAO6,Tic55-like4,TIC55-4 Pheophorbide a oxygenase-related protein; (1 of 8) 1.14.12.20 - Pheophorbide a oxygenase / Pheide a oxygenase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTTTTTGGAACGGATCCTCCATTATGCC
Internal bar code:GCCGTATTGTGTGATAGGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:842
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:755
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GACCAGACACGAAGAGAGCC
Suggested primer 2:AATGTAGCCATGCGGAAGTC