| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.201750 |
| Chromosome: | chromosome 1 |
| Location: | 4056942 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026500 | ASP3 | (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCCCCGATTGTCAGTGTATGAACCTT |
| Internal bar code: | TCGGTCCGGGCTTCGGGGTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 99.6211 |
| LEAP-Seq n confirming: | 5522 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGTTTGTATTGGTGCG |
| Suggested primer 2: | GCTTCTTCGACAACGCCTAC |