Insertion junction: LMJ.RY0402.201782_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.201782
Chromosome:chromosome 2
Location:1409527
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g083400 (1 of 1) PF07000 - Protein of unknown function (DUF1308) (DUF1308) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCACACACACACACACATACACACAC
Internal bar code:GGGATACAGATACGGAGGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:415
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:762
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGACGCATGCAAACAGACAC
Suggested primer 2:GTGGAGGTGAAGAACCAGGA